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|Título:||Regulatory role of Lactobacillus acidophilus on inflammation and gastric dysmotility in intestinal mucositis induced by 5‑fluorouracil in mice|
|Autor(es):||Justino, Priscilla F. C.|
Melo, Luis F. M.
Nogueira, Andre F.
Morais, Cecila M.
Mendes, Walber O.
Franco, Alvaro X.
Souza, Emmanuel P.
Ribeiro, Ronaldo A.
Souza, Marcellus H. L. P.
Soares, Pedro Marcos Gomes
|Data do documento:||Mar-2015|
|Editor:||Cancer Chemotherapy and Pharmacology|
|Citação:||JUSTINO, P. F. C. ; MELO, L. F. M. ; NOGUEIRA, A. F. ; MORAIS, C. M. ; MENDES, W. O. ; FRANCO, A. X. ; SOUZA, E. P. ; RIBEIRO, R. A. ; SOUZA, M. H. L. P. ; SOARES, P. M. G. (2015)|
|Abstract:||Purpose Lactobacillus acidophilus is widely used for gastrointestinal disorders, but its role in inflammatory con- ditions like in chemotherapy-induced mucositis is unclear. Here, we report the effect of L. acidophilus on 5-fluoroura- cil-induced (5-FU) intestinal mucositis in mice. Methods Mice weighing 25–30 g ( n = 8) were separated into three groups, saline, 5-FU, and 5-FU + L. acidophilus (5-FU-La) (16 × 10 9 CFU/kg). In the 5-FU-La group, L. acidophilus was administered concomitantly with 5-FU on the first day and alone for two additional days. Three days after the last administration of L. acidophilus , the animals were euthanized and the jejunum and ileum were removed for histopathological assessment and for evaluation of lev- els of myeloperoxidase activity, sulfhydryl groups, nitrite, and cytokines (TNF- α , IL-1 β , CXCL-1, and IL-10). In addition, we investigated gastric emptying using spectro- photometry after feeding a 1.5-ml test meal by gavage and euthanasia. Data were submitted to ANOVA and Bonfer - roni’s test, with the level of significance at p < 0.05. Results Intestinal mucositis induced by 5-FU significantly ( p < 0.05) reduced the villus height–crypt depth ratio and GSH concentration and increased myeloperoxidase activ- ity and the nitrite concentrations compared with the control group. Furthermore, 5-FU significantly ( p < 0.05) increased cytokine (TNF- α , IL-1 β , and CXCL-1) concentrations and decreased IL-10 concentrations compared with the con- trol group. 5-FU also significantly ( p < 0.05) delayed gas- tric emptying and gastrointestinal transit compared with the control group. All of these changes were significantly ( p < 0.05) reversed by treatment with L. acidophilus. Conclusions Lactobacillus acidophilus improves the inflammatory and functional aspects of intestinal mucositis induced by 5-FU.|
|Descrição:||JUSTINO, Priscilla F. C. ; MELO, Luis F. M. ; NOGUEIRA, André F. ; MORAIS, Cecíla M. ; MENDES, Walber O. ; FRANCO, Alvaro X. ; SOUZA, Emmanuel P. ; RIBEIRO, Ronaldo A. ; SOUZA, Marcellus H. L. P. ; SOARES, Pedro Marcos Gomes. Regulatory role of Lactobacillus acidophilus on inflammation and gastric dysmotility in intestinal mucositis induced by 5‑fluorouracil in mice. Cancer Chemotherapy and Pharmacology, Berlin, v. 75, n. 3, p. 559-567, mar. 2015.|
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|Aparece nas coleções:||DFIFA - Artigos publicados em revista científica|
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