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|Título:||Role of capsaicin-sensitive primary afferent neurons and non-protein sulphydryl groups on gastroprotective effect of amifostine against ethanol-induced gastric damage in rats|
Junqueira, Ana Flávia Torquato Araújo
Medeiros, Jand Venes R.
Barbosa, Sergio Henrique Brito
Nogueira, Ana Carolina Pereira
Mota, José Maurício Segundo Correia
Santana, Ana Paula Macêdo
Brito, Gerly Anne C.
Ribeiro, Ronaldo A.
Lima-Júnior, Roberto César P.
Souza, Marcellus H. L. P.
Células Receptoras Sensoriais
|Data do documento:||2011|
|Editor:||Digestive Diseases and Sciences|
|Citação:||JUNQUEIRA-JÚNIOR, J. ; JUNQUEIRA, A. F. T. A. ; MEDEIROS, J. V. R. ; BARBOSA, S. H. B. ; NOGUEIRA, A. C. P. ; MOTA, J. M. S. C. ; SANTANA, A. P. M. ; BRITO, G. A. C. ; RIBEIRO, R. A. ; LIMA-JÚNIOR, R. C. P. ; SOUZA, M. H. L. P. (2011)|
|Abstract:||Background Amifostine has been widely tested as a cytoprotective agent against a number of aggressors in different organs. Recently, a gastroprotective effect was observed for this drug in a model of indomethacin-induced gastric injury. Our objective was to investigate the effect of amifostine on ethanol-induced gastric injury and the role played in this mechanism by afferent sensory neurons, non- protein sulfhydryl groups, nitric oxide, ATP-sensitive potassium channels, and cyclooxygenase-2. Methods Rats were treated with amifostine (22.5, 45, 90, or 180 mg/kg, PO or SC). After 30 min, the rats received absolute ethanol (5 ml kg - 1 , PO). One hour later, gastric damage was quantified with a planimeter. Samples from the stomach were also taken for histopathological assessment and for assays of non-protein sulfhydryl groups. The other groups were pretreated with L-NAME (10 mg kg - 1 , IP), glibenclamide (10 mg kg - 1 , PO), or celecoxib (10 mg kg - 1 , PO). After 30 min, the animals were given amifostine (90 mg kg - 1 , PO or SC), followed 30 min later by gavage with absolute ethanol (5 ml kg - 1 ). Other rats were desen- sitized with capsaicin (125 mg kg - 1 , SC) 8 days prior to amifostine treatment. Results Amifostine administration PO and SC significantly and dose-dependently reduced ethanol-induced macroscopic and microscopic gastric damage by restoring glutathione levels in the stomach mucosa. Amifostine-promoted gas- troprotection against ethanol-induced stomach injury was reversed by pretreatment with neurotoxic doses of capsaicin, but not by L-NAME, glibenclamide, or celecoxib. Conclusions Amifostine protects against ethanol-induced gastric injury by increasing glutathione levels and stimu- lating the afferent sensory neurons in the stomach.|
|Descrição:||JUNQUEIRA-JÚNIOR, Jerônimo J ; JUNQUEIRA, Ana Flávia Torquato Araújo J; MEDEIROS, Jand Venes R. ; BARBOSA, Sergio Henrique Brito ; NOGUEIRA, Ana Carolina Pereira ; MOTA, José Maurício Segundo Correia ; SANTANA, Ana Paula Macêdo ; BRITO, Gerly Anne C. ; RIBEIRO, Ronaldo A. ;LIMA-JÚNIOR, Roberto César P. ; SOUZA, Marcellus H. L. P. Role of capsaicin-sensitive primary afferent neurons and non-protein sulphydryl groups on gastroprotective effect of amifostine against ethanol-induced gastric damage in rats. Digestive Diseases and Sciences, New York, v. 56, p. 314-22, 2011.|
1573-2568 On line
|Aparece nas coleções:||DFIFA - Artigos publicados em revista científica|
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