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dc.contributor.authorBrilhante, Raimunda Sâmia Nogueira-
dc.contributor.authorSilva, Natalya Fechine-
dc.contributor.authorLima, Rita Amanda Chaves de-
dc.contributor.authorCaetano, Érica Pacheco-
dc.contributor.authorAlencar, Lucas Pereira de-
dc.contributor.authorCastelo-Branco, Débora de Souza Collares Maia-
dc.contributor.authorMoreira, José Luciano Bezerra-
dc.contributor.authorBandeira, Silviane Praciano-
dc.contributor.authorCamargo, Zoilo Pires de-
dc.contributor.authorRodrigues, Anderson Messias-
dc.contributor.authorBandeira, Tereza de Jesus Pinheiro Gomes-
dc.contributor.authorMonteiro, André Jalles-
dc.contributor.authorCordeiro, Rossana de Aguiar-
dc.contributor.authorSidrim, José Júlio Costa-
dc.contributor.authorRocha, Marcos Fábio Gadelha-
dc.date.accessioned2016-06-23T12:22:00Z-
dc.date.available2016-06-23T12:22:00Z-
dc.date.issued2015-04-
dc.identifier.citationBRILHANTE, R. S. N. et al. Easy storage strategies for sporothrix spp. strains. Biopreservation and Biobanking, v. 13, n. 2, p. 131-134, apr. 2015.pt_BR
dc.identifier.issn1947-5535-
dc.identifier.urihttp://www.repositorio.ufc.br/handle/riufc/17895-
dc.description.abstractThe present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis;6S. schenckii;5 S. mexicana, and 3 S. globosa) in saline at 4 C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 C and -80 C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 C and at -20 C did not alter the number of conidia, but lower conidium counts were observed at -80 C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.pt_BR
dc.language.isoenpt_BR
dc.publisherBiopreservation and Biobankingpt_BR
dc.subjectSporothrixpt_BR
dc.subjectGlicerolpt_BR
dc.subjectLactosept_BR
dc.titleEasy storage strategies for sporothrix spp. strainspt_BR
dc.typeArticlept_BR
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