Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/18663
Título: Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X : in vivo and ex vivo methods and microscopy examinations
Autor(es): Ferreira, Paulo Michel Pinheiro
Bezerra, Daniel Pereira
Silva, Jurandy do Nascimento
Costa, Marcília Pinheiro da
Ferreira, José Roberto de Oliveira
Alencar, Nylane Maria Nunes
Figueiredo, Ingrid Samantha Tavares de
Cavalheiro, Alberto José
Machado, Camila Maria Longo
Chammas, Roger
Alves, Ana Paula Negreiros Nunes
Moraes, Manoel Odorico de
Pessoa, Claudia
Palavras-chave: Sarcoma 180
Antineoplásicos
Toxicidade
Data do documento: Jun-2016
Editor: Journal of Ethnopharmacology
Citação: FERREIRA, P. M. P. ; BEZERRA, D. P. ; SILVA, J. N. ; COSTA, M. P. ; FERREIRA, J. R. O. ; ALENCAR, N. M. N. ; FIGUEIREDO, I. S. T. ; CAVALHEIRO, A. J. ; MACHADO, C. M. L. ; CHAMMAS, R. ; ALVES, A. P. N. N. ; MORAES, M. O. ; PESSOA, C. (2016)
Abstract: Ethnopharmacological relevance Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim of the study To assess the in vivo and ex vivo antitumor action of a fraction with casearins (FC) and its main component - Casearin X-isolated from C. sylvestris leaves. Materials and methods Firstly, Sarcoma 180 bearing Swiss mice were treated with FC and Cas X for 7 days. Secondly, BALB/c nude animals received hollow fibers with colon carcinoma (HCT-116) or glioblastoma (SF-295) cells and were treated with FC for 4 days. On 5th day, proliferation was determined by MTT assay. Results FC 10 and 25 mg/kg/day i.p. and 50 mg/kg/day oral and Cas X 25 mg/kg/day i.p. and 50 mg/kg/day oral revealed tumor growth inhibition rates of 35.8, 86.2, 53.7, 90.0 and 65.5% and such tumors demonstrated rare mitoses and coagulation necrosis areas. Similarly, FC reduced multiplying of HCT-116 and SF-295 cells when evaluated by the Hollow Fiber Assay (2.5 and 5 mg/kg/day i.p. and 25 and 50 mg/kg/day oral), with cell growth inhibition rates ranging from 33.3 to 67.4% (p<0.05). Flow cytometry experiments revealed that FC reduced membrane integrity and induced DNA fragmentation and mitochondrial depolarization (p<0.05). Conclusions FC and Cas X were efficient antitumor substances against murine and human cancer cells and caused reversible morphological changes in liver, kidneys and spleens, emphasizing clerodane diterpenes as an emerging class of anticancer molecules.
Descrição: FERREIRA, Paulo Michel Pinheiro et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X : in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270-279, jun., 2016.
URI: http://www.repositorio.ufc.br/handle/riufc/18663
ISSN: 0378-8741
1872-7573
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