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|Título:||Prolonged maternal separation induces undernutrition and systemic inflammation with disrupted hippocampal development in mice|
|Autor(es):||Figueiredo, Ítalo Leite|
Frota, Priscila B.
Cunha, Davi G. da
Raposo, Ramon da Silva
Canuto, Kildere M.
Andrade, Geanne M. de
Moore, Sean R.
Anstead, Gregory M.
Alvarez-Leite, Jacqueline I.
Guerrant, Richard L.
Oriá, Reinaldo B.
|Data do documento:||Set-2016|
|Citação:||FIGUEIREDO, Í. L. ; FROTA, P. B. ; CUNHA, D. G. ; RAPOSO, R. S. ; CANUTO, K. M. ; ANDRADE, G. M. ; SOUSA, N. ; MOORE, S. R. ; ANSTEAD, G. M. ; ALVAREZ-LEITE, J. I. ; GUERRANT, R. L. ; ORIÁ, R. B. (2016)|
|Abstract:||Objective Prolonged maternal separation (PMS) in the first 2 wk of life has been associated with poor growth with lasting effects in brain structure and function. This study aimed to investigate whether PMS-induced undernutrition could cause systemic inflammation and changes in nutrition-related hormonal levels, affecting hippocampal structure and neurotransmission in C57BL/6J suckling mice. Methods This study assessed mouse growth parameters coupled with insulin-like growth factor-1 (IGF-1) serum levels. In addition, leptin, adiponectin, and corticosterone serum levels were measured following PMS. Hippocampal stereology and the amino acid levels were also assessed. Furthermore, we measured myelin basic protein and synapthophysin (SYN) expression in the overall brain tissue and hippocampal SYN immunolabeling. For behavioral tests, we analyzed the ontogeny of selected neonatal reflexes. PMS was induced by separating half the pups in each litter from their lactating dams for defined periods each day (4 h on day 1, 8 h on day 2, and 12 h thereafter). A total of 67 suckling pups were used in this study. Results PMS induced significant slowdown in weight gain and growth impairment. Significant reductions in serum leptin and IGF-1 levels were found following PMS. Total CA3 area and volume were reduced, specifically affecting the pyramidal layer in PMS mice. CA1 pyramidal layer area was also reduced. Overall hippocampal SYN immunolabeling was lower, especially in CA3 field and dentate gyrus. Furthermore, PMS reduced hippocampal aspartate, glutamate, and gamma-aminobutyric acid levels, as compared with unseparated controls. Conclusion These findings suggest that PMS causes significant growth deficits and alterations in hippocampal morphology and neurotransmission.|
|Descrição:||FIGUEIREDO, Ítalo Leite et al. Prolonged maternal separation induces undernutrition and systemic inflammation with disrupted hippocampal development in mice. Nutrition, v. 32, n. 9, p. 1019–1027, sep., 2016.|
|Aparece nas coleções:||DFIFA - Artigos publicados em revista científica|
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