Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/19256
Título: Lectin from Abelmoschus esculentus reduces zymosan-induced temporomandibular joint inflammatory hypernociception in rats via heme oxygenase-1 pathway integrity and tnf-α and il-1β suppression
Autor(es): Freitas, Raul Sousa
Val, Danielle Rocha do
Fernandes, Maria Ester Frota
Gomes, Francisco Isaac Fernandes
Lacerda, José Thalles Jocelino Gomes de
Gadelha, Tatiane Santi
Gadelha, Carlos Alberto de Almeida
Pinto, Vicente de Paulo Teixeira
Pereira, Karuza Maria Alves
Brito, Gerly Anne de Castro
Bezerra, Mirna Marques
Chaves, Hellíada Vasconcelos
Cristino-Filho, Gerardo
Palavras-chave: Abelmoschus
Articulação Temporomandibular
Temporomandibular Joint
Data do documento: Set-2016
Editor: International Immunopharmacology
Citação: FREITAS, R. S. ; VAL, D. R. ; FERNANDES, M. E. F. ; GOMES, F. I. F. ; LACERDA, J. T. J. G. ; GADELHA, T. S. ; GADELHA, C. A. A. ; PINTO, V. P. T. ; CRISTINO-FILHO, G. ; PEREIRA, K. M. A. ; BRITO, C. G. A. ; BEZERRA, M. M. ; CHAVES, H. V. (2016)
Abstract: Temporomandibular joint (TMJ) disorders show inflammatory components, heavily impacting on quality of life. Abelmoschus esculentus is largely cultivated in Northeastern Brazil for medicinal purposes, having it shown anti-inflammatory activity. We evaluated A. esculentus lectin (AEL) efficacy in reducing zymosan-induced temporomandibular joint inflammatory hypernociception in rats along with the mechanism of action through which it exerts anti-inflammatory activity. Animals were pre-treated with AEL (0.01, 0.1 or 1 mg/kg) before zymosan (Zy) injection in the TMJ to determine anti-inflammatory activity. To analyse the possible effect of the hemeoxygenase-1 (HO-1) and the nitric oxide (NO) pathways on AEL efficacy, animals were pre-treated with ZnPP-IX (3 mg/kg), a specific HO-1 inhibitor, or aminoguanidine (30 mg/kg), a selective iNOS inhibitor, before AEL administration. Von Frey test evaluated inflammatory hypernociception, synovial fluid collection was performed to determine leukocyte counting and myeloperoxidase (MPO) activity 6 h after Zy injection, and Evans Blue extravasation determined vascular permeability. TMJ tissue was collected for histopathological analysis (H&E) and immunohistochemistry (TNF-α, IL-1β, HO-1). In addition, TMJ tissue and trigeminal ganglion collection was performed for TNF-α and IL-1β dosage (ELISA). AEL increased inflammatory nociceptive threshold, reduced leukocyte influx along with MPO activity, leukocyte influx into the synovial membrane, and Evans Blue extravasation. It promoted HO-1 overexpression whilst decreased TNF-α and IL-1β expression in the TMJ tissue. AEL reduced TNF-α and IL-1β levels in TMJ tissue and trigeminal ganglion. AEL effects, however, were not observed in the presence of ZnPP-IX. These findings suggest that AEL efficacy depends on TNF-α/IL-1β inhibition and HO-1 pathway integrity.
Descrição: FREITAS, Raul Sousa et al. Lectin from Abelmoschus esculentus reduces zymosan-induced temporomandibular joint inflammatory hypernociception in rats via heme oxygenase-1 pathway integrity and tnf-α and il-1β suppression. International Immunopharmacology, v. 38, p. 313-323, sep., 2016.
URI: http://www.repositorio.ufc.br/handle/riufc/19256
ISSN: 1567-5769
1878-1705
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