Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/5279
Title in Portuguese: PCR-REA as an important tool for the identification of Cryptococcus neoformans and Cryptococcus gattii from human and veterinary sources
Author: Cordeiro, Rossana Aguiar
Costa, Ana Karoline Freire
Brilhante, Raimunda Sâmia Nogueira
Lima, Rita Amanda Chaves de
Castelo-Branco, Débora de Souza Collares Maia
Ribeiro, Joyce Fonteles
Monteiro, André Jalles
Rocha, Francisco Airton Castro
Sidrim, José Júlio Costa
Rocha, Marcos Fábio Gadelha
Keywords: Cryptococcus neoformans
Cryptococcus gattii
Issue Date: Dec-2011
Publisher: Veterinary Microbiology
Citation: CORDEIRO, R. A. et al. PCR-REA as an important tool for the identification of Cryptococcus neoformans and Cryptococcus gattii from human and veterinary sources. Veterinary Microbiology, Amsterdam, v. 1, n. 1-2, p. 180-184, dez. 2011.
Abstract: The extraordinary ability of Cryptococcus species to cause disease has focused the attention of scientists on finding ways to improve their identification methods. In this study, PCR- REA, manual methods (morphological and biochemical characteristics), API 20C and VITEK 2 were used to test identify a total of 30 Cryptococcus spp. from human and veterinary sources. PCR-REA was performed using the capsular region as amplification target followed by restriction with the enzymes Age I, Bsm FI and Hpa II. PCR-REA identified the strains as C . neoformans var. grubii ( n = 19) and C . gattii ( n = 8). There was no significant difference between the API 20C AUX and VITEK 2 when compared to manual methods for the identification of Cryptococcus spp. However, none of these non-manual methods were able to detect C. gattii samples. PCR-REA showed a greater level of concordance with the manual method, besides being faster and more sensitive than the other methods. Therefore, it is indicated for routine identification of Cryptococcus spp. strains.
URI: http://www.repositorio.ufc.br/handle/riufc/5279
metadata.dc.type: Artigo de Periódico
ISSN: 0378-1135
Appears in Collections:DMC - Artigos publicados em revistas científicas

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