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Título: Inactivation of COX-2 , HMLH1 and CDKN2A gene by promoter methylation in gastric cancer : relationship with histological subtype, tumor location and helicobacter pylori genotype
Autor(es): Alves, Markênia Kélia Santos
Ferrasi, Adriana Camargo
Lima, Valeska Portela
Ferreira, Márcia Valéria Pitombeira
Pardini, Maria Inês de Moura Campos
Rabenhorst, Silvia Helena Barem
Palavras-chave: Neoplasias Gástricas
Metilação
Helicobacter pylori
Data do documento: Out-2011
Editor: Pathobiology
Citação: ALVES, M. K. S. ; FERRASI, A. C. ; LIMA, V. P. ; FERREIRA, M. V. P. ; PARDINI, M. I. M. C. ; RABENHORST, S. H. B. (2011)
Abstract: Objective: We aimed to evaluate the inactivation of COX-2 , HMLH1 and CDKN2A by promoter methylation and its rela- tionship with the infection by different Helicobacter pylori strains in gastric cancer. Methods: DNA extracted from 76 H. pylori -positive gastric tumor samples was available for pro- moter methylation identification by methylation-specific PCR and H. pylori subtyping by PCR. Immunohistochemistry was used to determine COX-2, p16 INK4A and HMLH1 expres- sion. Results: A strong negative correlation was found be- tween the expression of these markers and the presence of promoter methylation in their genes. Among cardia tumors, negativity of p16 INK4A was a significant finding. On the other hand, in noncardia tumors, th e histological subtypes had different gene expression patterns. In the intestinal subtype, a significant finding was HMLH1 inactivation by methylation, while in the diffuse subtype, CDKN2A inactivation by meth- ylation was the significant finding. Tumors with methylated COX-2 and HMLH1 genes were associated with H. pylori vacA s1 (p = 0.025 and 0.047, respec tively), and the nonmethylated tumors were associated with the presence of the gene fla A. Conclusions: These data suggest that the inactivation of these genes by methylation occurs by distinct pathways ac- cording to the histological subtype and tumor location and depends on the H. pylori genotype.
Descrição: ALVES, Markênia Kélia Santos; FERRASI, Adriana Camargo; LIMA, Valeska Portela; FERREIRA, Márcia Valéria Pitombeira; PARDINI, Maria Inês de Moura Campos; RABENHORST, Silvia Helena Barem. Inactivation of COX-2, HMLH1 and CDKN2A gene by promoter methylation in gastric cancer : relationship with histological subtype, tumor location and Helicobacter pylori genotype. Pathobiology, Basel, v. 78, n. 5, p. 266-276, ago. 2011.
URI: http://www.repositorio.ufc.br/handle/riufc/5340
ISSN: 1015-2008
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