Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/5369
Título: CDKN2A promoter methylation is related to the tumor location and histological subtype and associated with Helicobacter pylori fla A(+) strains in gastric adenocarcinomas
Autor(es): Alves, Markênia Kélia Santos
Lima, Valeska Portela
Ferrasi, Adriana Camargo
Rodrigues, Maria Aparecida
Pardini, Maria Inês de Moura Campos
Rabenhorst, Silvia Helena Barem
Palavras-chave: Helicobacter pylori
Neoplasias Gástricas
Metilação
Data do documento: Abr-2010
Editor: APMIS. Acta Pathologica, Microbiologica et Immunologica Scandinavica
Citação: ALVES, M. K. S. ; LIMA, V. P. ; FERRASI, A. C. ; RODRIGUES, M. A. ; PARDINI, M. I. M. C. ; RABENHORST, S. H. B. (2010)
Abstract: Promoter hypermethylation of CDKN2A (p16 INK4A protein) is the main mechanism of gene inactiva- tion. However, its association with Helicobacter pylori infection is a controversial issue. Therefore, we examined a series of gastric adenocarcinomas to assess the association between p16 INK4A inactivation and H. pylori genotype ( va cA, cag A, cag E, vir B11 and fla A) according to the location and histological subtype of the tumors. p16 INK4A expression and CDKN2A promoter methylation were found in 77 gas- tric adenocarcinoma samples by immunohistochemistry and methylation-specific PCR, respectively. Helicobacter pylori infection and genotype were determined by PCR. A strong negative correlation between immunostaining and CDKN2A promoter region methylation was found. In diffuse subtype tumors, the inactivation of p16 INK4A by promoter methylation was unique in noncardia tumors (p = 0.022). In addition, H. pylori -bearing fla A was associated with non-methylation tumors (p = 0.008) and H. pylori strain bearing cag Aor vac As1m1 genes but without fla A was associated with methylated tumors (p = 0.022 and 0.003, respectively). Inactivation of p16 INK4A in intestinal and diffuse subtypes showed distinct carcinogenic pathways, depending on the tumor location. Moreover, the process of methylation of the CDKN2A promoter seems to depend on the H. pylori genotype. The present data suggest that there is a differential influence and relevance of H. pylori genotype in gastric cancer development
Descrição: ALVES, Markênia Kélia Santos; LIMA, Valeska Portela; FERRASI, Adriana Camargo; RODRIGUES, Maria Aparecida; PARDINI, Maria Inês de Moura Campos; RABENHORST, Silvia Helena Barem. CDKN2A promoter methylation is related to the tumor location and histological subtype and associated with A(+) strains in gastric adenocarcinomas. APMIS. Acta Pathologica, Microbiologica et Immunologica Scandinavica, v. 118, n. 4, p. 297-307, abr. 2010.
URI: http://www.repositorio.ufc.br/handle/riufc/5369
ISSN: 0903-4641
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