Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/5380
Title in Portuguese: MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
Author: Neves Filho, Eduardo Henrique Cunha
Alves, Markenia Kelia Santos
Lima, Valeska Portela
Rabenhorst, Silvia Helena Barem
Keywords: Helicobacter pylori
Neoplasias Gástricas
Issue Date: Dec-2010
Publisher: Virchows Archiv
Citation: NEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010.
Abstract: Abstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strains
URI: http://www.repositorio.ufc.br/handle/riufc/5380
metadata.dc.type: Artigo
ISSN: 1432-2307 Online
0945-6317 Impressa
Appears in Collections:DMC - Artigos publicados em revistas científicas

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