Use este identificador para citar ou linkar para este item: http://repositorio.ufc.br/handle/riufc/54419
Tipo: Artigo de Evento
Título: Expression and purification of a new esterase from metagenomic clone similar to bacterial esterase of the Porticoccus hydrocarbonoclasticus
Autor(es): Araújo, Francisco Jonathan dos Santos
Gomes, Brenda Suellen Rodrigues
Bessa, Claudiane Carvalho
Soares Júnior, João Alberto de Oliveira
Hissa, Denise Cavalcante
Melo, Vânia Maria Maciel
Palavras-chave: Esterases;Esterase LipG7;Enzimas
Data do documento: 2018
Citação: ARAÚJO, Francisco Jonathan dos Santos; GOMES, Brenda Suellen Rodrigues; BESSA, Claudiane Carvalho; SOARES JÚNIOR, João Alberto de Oliveira; HISSA, Denise Cavalcante; MELO, Vânia Maria Maciel. Expression and purification of a new esterase from metagenomic clone similar to bacterial esterase of the Porticoccus hydrocarbonoclasticus. In: CONGRESSO BRASILEIRO DE ENGENHARIA QUÍMICA, XXII., 23 a 26 set. 2018; ENCONTRO BRASILEIRO SOBRE O ENSINO DE ENGENHARIA QUÍMICA, XVII., 27 a 28 set. 2018, São Paulo, Brasil. Anais […] São Paulo, 2018.
Abstract: Esterases are lipolytic enzymes widely used in industrial applications. This work aimed the overexpression and purification of the esterase LipG7 identified from a metagenomic library constructed from mangrove sediments, which gene sequence shares 80% amino acid identity to 1,4-butanediol diacrylate esterase from the bacterium Porticoccus hydrocarbonoclasticus. LipG7 was expressed in three commercial Escherichia coli strains, Rosetta-gami, ArcticExpress and BL21 and the activity evaluated against 4-nitrophenyl butyrate substract. Recombinante esterase was obtained in soluble form only in Rosetta-gami after treatment with guanidine hydrochloride. It was active against 4-nitrophenyl butyrate at 30 °C with specific activity of 216.3 ± 16.4 U/mg that was significantly enhanced in presence of Mg2+ ion.
URI: http://www.repositorio.ufc.br/handle/riufc/54419
ISSN: 2359-1757
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