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dc.contributor.authorNogueira, Fábio C. S.-
dc.contributor.authorPalmisano, Giuseppe-
dc.contributor.authorSoares, Emanoella L.-
dc.contributor.authorShah, Mohibullah-
dc.contributor.authorSoares, Arlete A.-
dc.contributor.authorRoepstorff, Peter-
dc.contributor.authorCampos, Francisco A. P.-
dc.contributor.authorDomont, Gilberto B.-
dc.identifier.citationNOGUEIRA, Fábio C. S. et al. Proteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during development. Journal Of Proteomics, [s.l.], v. 75, p. 1933-1939, 2012.pt_BR
dc.description.abstractIn this study, we performed a proteomic analysis of nucellus from two developmental stages of Ricinus communis seeds by a GeLC-MS/MS approach, using of a high resolution orbi trap mass spectrometer, which resulted in the identification of a total of 766 proteins that were grouped into 553 protein groups. The distribution of the identified proteins in stages III and IV into different Gene Ontology categories was similar, with a remarkable abundance of proteins associated with the protein synthesis machinery of cells, as well as several clas ses of proteins involved in protein degradation, particularly of peptidases associated with programmed cell death. Consistent with the role of the nucellus in mediating nutrient transfer from maternal tissues to the endosperm and embryo, a significant proportion of the identified proteins are related to amino acid metabolism, but none of the identified pro teins are known to have a role as storage proteins. Moreover for the first time, ricin isoforms were identified in tissues other than seed endosperm. Results are discussed in the context of the spatial and temporal distribution of the identified proteins within the nucellar cell layers.pt_BR
dc.publisherJournal Of Proteomicspt_BR
dc.subjectRicinus communispt_BR
dc.subjectCastor beanpt_BR
dc.subjectOil seedpt_BR
dc.subjectPlant proteomicspt_BR
dc.subjectProgrammed cell deathpt_BR
dc.titleProteomic profile of the nucellus of castor bean (Ricinus communis L.) seeds during developmentpt_BR
dc.typeArtigo de Periódicopt_BR
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