Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/8343
Title in Portuguese: Histological study of rat ovaries cryopreserved by vitrification or slow freezing and reimplanted in the early or late postmenopausal stage
Author: Silva, João Marcos de Meneses e
Pinheiro, Luiz Gonzaga Porto
Leite, José Alberto Dias
Melo, Lígia Helena Ferreira
Lunardi, Franciele Osmarini
Barbosa Filho, Rômulo Cesar Costa
Mendonça, Cindy Vitalino
Keywords: Criopreservação
Vitrificação
Ovariectomia
Issue Date: 2014
Publisher: Acta Cirúrgica Brasileira
Citation: SILVA, J. M. de M. e et al. Histological study of rat ovaries cryopreserved by vitrification or slow freezing and reimplanted in the early or late postmenopausal stage. Acta Cirúrgica Brasileira, São Paulo, SP, v. 29, n. 5, p. 299-305, 2014.
Abstract: PURPOSE: To compare two rat ovary cryopreservation techniques (vitrification vs. slow freezing) and two postmenopausal stages (early vs. late) with regard to graft take.METHODS: Thirty-three Wistar rats were submitted to bilateral oophorectomy. One ovary was submitted to histological analysis while the other was cryopreserved by slow freezing or vitrification. The cryopreserved ovary was thawed and reimplanted in the greater omentum one week (early menopause) or one month (late menopause) after oophorectomy. One month after ovary reimplantation, the graft take was evaluated macroscopically and histologically. RESULTS: Six of the animals were used ascontrols and seven died. The histological findings of 20 animals included atretic follicles (n=4), primordial follicles (n=2), and corpus luteum with primordial follicles (n=3). No ovarian tissue was found in 11 animals. Vitrification resulted in a higher graft take rate than slow freezing (50% vs. 38.5%), but the difference was not statistically significant. However, the graft take rate was 9.3 times higher in the early than in the late postmenopausal stage (61.5% vs. 14.3%) (p=0.043).CONCLUSION: Vitrification was superior to slow freezing as ovarian cryopreservation technique, and grafting was significantly more successful when the ovary was reimplanted in the late postmenopausal stage.
URI: http://www.repositorio.ufc.br/handle/riufc/8343
metadata.dc.type: Artigo de Periódico
ISSN: 0102-8650
Appears in Collections:DCIR - Artigos publicados em revista científica

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