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|Title in Portuguese:||Farnesol increases the susceptibility of Burkholderia pseudomallei bioﬁlm to antimicrobials used to treat melioidosis|
|Author:||Castelo-Branco, D. S. C. M.|
Riello, G. B.
Vasconcelos, D. C.
Guedes, G. M. M.
Bandeira, T. J. P. G.
Monteiro, A. J.
Cordeiro, R. A.
Rocha, M. F. G.
Sidrim, J. J. C.
Brilhante, R. S. N.
|Publisher:||Journal of Applied Microbiology|
|Citation:||CASTELO-BRANCO, D.S.C.M. et al. Farnesol increases the susceptibility of Burkholderia pseudomallei biofilm to antimicrobials used to treat melioidosis. Journal of Applied Microbiology, v. 120, p. 600-606, 2015.|
|Abstract:||Aims: The aim of this study was to analyse the in vitro activity of farnesol alone and combined with the antibacterial drugs amoxicillin, doxycycline, ceftazidime and sulfamethoxazole – trimethoprim against Burkholderia pseudomallei bioﬁlms. Methods and Results: Susceptibility was assessed by the broth microdilution test and cell viability was re ad with the oxid ation–reduction indicator dye resazurin. The bioﬁlms were evaluated through three microscopic techniques (optical, confocal and electronic microscopy). The minimum bioﬁlm erradication concentration (MBEC) for farnesol was 75–2400 mmol l 1 . In addition, farnesol signiﬁcantly reduced the MBEC values for ceftazidime, amoxicillin, doxycycline and sulfamethoxazole–trimethoprim by 256, 16, 4 and 4 times respectively (P < 005). Optical, confocal and electronic microscopic analyses of farnesol-treated B. pseudomallei bioﬁlms demonstrated that this compound damages bioﬁlm matrix, probably facilitating antimicrobial pe netration in the bioﬁ lm stru cture. Conclusions: This study demonstrated the effectiveness of farnesol against B. pseudomallei bioﬁlms and its potentiating effect on the activity of antibacterial drugs, in particular ceftazidime, amoxicillin, doxycycline and sulfamethoxazole–trimethoprim. Signiﬁcance and Impact of the Study: The intrinsic antimicrobial resistance of B. pseudomallei is a serious challenge for the treatment of melioidosis. Thus, this paper reports the inhibitory potential of farnesol against B. pseudomallei bioﬁlms, as well as highlights the favourable pharmacological interaction of farnesol with antibiotics tested, not only on cell viability, but also in the structural morphology of bioﬁlms.|
|metadata.dc.type:||Artigo de Periódico|
|Appears in Collections:||DPML - Artigos publicados em revista científica|
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