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Título: The major sterols of Sargassum vulgare C. Agardh investigated by mass chromatography
Autor(es): Halket, John M.
Joventino, Francisca Pinheiro
Lisboa, Belizário P.
Palavras-chave: Alga
Data do documento: 1976
Editor: Arquivos de Ciências do Mar
Citação: HALKET, J. M.; JOVENTINO, F. P. ; LISBOA, B. P. (1976)
Resumo: Increasing interest is being shown in the sterol contents of marine plants (Patterson, 1971; Goodwin, 1974) and invertebrates (Patterson et al., 1975) for such reasons as chemotaxonomic significance, economics and diet. The recent introduction of more effective analytical methods in this área e.g. gás chromatography mass spectrometry and hydrophobic gel column chromatography (Patterson et al., 1975) are helping to reduce many problems concerning sensitivity, specificity and separating powers which beset earlier workers. It is generally recognized that the brown algae, Phaeophyceae, contain fucosterol as the major sterol component (Ikekawa et al.1968; Patterson, 1971; Goodwin, 1974). Unless otherwise stated in the text ali reagents used were of analytical grade obtained from E. Merck A.G., Darmstadt. Fucosterol (5,24(28) E-cholestadíene-3" - ol) was obtained from Steraloids Inc., Wilton N.H., U.S.A. and 28-iso-fucosterol (5,24(28) Z-cholestadiene-3 * ol) was a gift from Prof. WSucrow, Paderborn. Sargassum vulgare was harvested near Fortaleza, Brazil during the month of July. An ethanolic extract was made by grinding 200 g of clean wet alga with 100 ml of distilled water and 30 ml of ethanol. The resulting mixture was filtered, evaporated to dryness (75°-80°C), made up to 160 ml with ethanol and stored in a refrigerator. From this solution 40 ml were taken, evaporated to dryness and made up to 200 ml with distilled water.
Descrição: HALKET, John M.; JOVENTINO, Francisca Pinheiro; LISBOA, Belizário P. The major sterols of Sargassum vulgare C. Agardh investigated by mass chromatography. Arquivos de Ciências do Mar. Fortaleza, v.16, n.2, p.117-122, dez. 1976.
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