Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/16959
Título: Cytotoxic and toxicological effects of phthalimide derivatives on tumor and normal murine cells
Autor(es): Ferreira, Paulo Michel Pinheiro
Costa, Patrícia Marçal da
Costa, Arinice de Menezes
Lima, Daisy Jereissati Barbosa
Drumond, Renata Rosado
Silva, Jurandy do Nascimento
Moreira, Diogo Rodrigo de Magalhães
Oliveira Filho, Gevânio Bezerra de
Ferreira, Jamile Magalhães
Queiroz, Maria Goretti Rodrigues de
Leite, Ana Cristina Lima
Pessoa, Cláudia
Palavras-chave: Sarcoma 180
Citotoxinas
Data do documento: 2015
Editor: Anais da Academia Brasileira de Ciências
Citação: FERREIRA, P. M. P. ; COSTA, P. M. ; COSTA, A. M. ; LIMA, D. J. B. ; DRUMOND, R. R. ; SILVA, J. N. ; MOREIRA, D. R. M. ; OLIVEIRA FILHO, G. B. ; FERREIRA, J. M. ; QUEIROZ, M. G. R. ; LEITE, A. C. L. ; PESSOA, C. (2015)
Abstract: Eleven phthalimide derivatives were evaluated with regards to their antiproliferative activity on tumor and normal cells and possible toxic effects. Cytotoxic analyses were performed against murine tumors (Sarcoma 180 and B-16/F-10 cells) and peripheral blood mononuclear cells (PBMC) using MTT and Alamar Blue assays. Following, the investigation of cytotoxicity was executed by flow cytometry analysis and antitumoral and toxicological potential by in vivo techniques. The molecules 3b, 3c, 4 and 5 revealed in vitro cytotoxicity against Sarcoma 180, B-16/F-10 and PBMC. Since compound 4 was the most effective derivative, it was chosen to detail the mechanism of action after 24, 48 and 72 h exposure (22.5 and 45 μM). Sarcoma 180 cells treated with compound 4 showed membrane disruption, DNA fragmentation and mitochondrial depolarization in a time- and dose-dependent way. Compounds 3c, 4 and 5 (50 mg/kg/day) did not inhibit in vivo tumor growth. Compound 4-treated animals exhibited an increase in total leukocytes, lymphocytes and spleen relative weight, a decreasing in neutrophils and hyperplasia of spleen white pulp. Treated animals presented reversible histological changes. Molecule 4 had in vitro antiproliferative action possibly triggered by apoptosis, reversible toxic effects on kidneys, spleen and livers and exhibited immunostimulant properties that can be explored to attack neoplasic cells.
Descrição: FERREIRA, Paulo Michel Pinheiro ; COSTA, Patricia Marçal da ; COSTA, Arinice de Menezes ; LIMA, Daisy Jereissati Barbosa ; DRUMOND, Renata Rosado. ; SILVA, Jurandy do Nascimento ; MOREIRA, Diogo Rodrigo de Magalhães ; OLIVEIRA FILHO, Gevânio Bezerra de ; FERREIRA, Jamile Magalhães ; QUEIROZ, Maria Goretti Rodrigues de ; LEITE, Ana Cristina Lima ; PESSOA, Cláudia. Cytotoxic and toxicological effects of phthalimide derivatives on tumor and normal murine cells. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 87, n. 1, p. 313-330.
URI: http://www.repositorio.ufc.br/handle/riufc/16959
ISSN: 1678-2690 On line
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