Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/17731
Título: Deregulated expression of SRC, LYN and CKB kinases by DNA methylation and its potential role in gastric cancer Invasiveness and metastasis
Autor(es): Mello, Adriano Azevedo
Leal, Mariana Ferreira
Rey, Juan Antonio
Pinto, Giovanny Rebouças
Lamarão, Leticia Martins
Montenegro, Raquel Carvalho
Alves, Ana Paula Negreiros Nunes
Assumpção, Paulo Pimentel
Borges, Barbara do Nascimento
Smith, Marília Cardoso
Burbano, Rommel Rodriguez
Palavras-chave: Neoplasias Gástricas
DNA
Data do documento: Out-2015
Editor: Plos One
Citação: MELLO, A. A. ; LEAL, M. F. ; REY, J. A. ; PINTO, G. R. ; LAMARÃO, L. M. ; MONTENEGRO, R. C. ; ALVES, A. P. N. N. ; ASSUMPÇÃO, P. P. ; BORGES, B. N. ; SMITH, M. C. ; BURBANO, R. R. (2015)
Abstract: Kinases are downstream modulators and effectors of several cellular signaling cascades and play key roles in the development of neoplastic disease. In this study, we aimed to evaluate SRC, LYN and CKB protein and mRNA expression, as well as their promoter methylation, in gastric cancer. We found elevated expression of SRC and LYN kinase mRNA and protein but decreased levels of CKB kinase, alterations that may have a role in the invasiveness and metastasis of gastric tumors. Expression of the three studied kinases was also associated with MYC oncogene expression, a possible biomarker for gastric cancer. To understand the mechanisms that regulate the expression of these genes, we evaluated the DNA promoter methylation of the three kinases. We found that reduced SRC and LYN methylation and increased CKB methylation was associated with gastric cancer. The reduced SRC and LYN methylation was associated with increased levels of mRNA and protein expression, suggesting that DNA methylation is involved in regulating the expression of these kinases. Conversely, reduced CKB methylation was observed in samples with reduced mRNA and protein expression, suggesting CKB expression was found to be only partly regulated by DNA methylation. Additionally, we found that alterations in the DNA methylation pattern of the three studied kinases were also associated with the gastric cancer onset, advanced gastric cancer, deeper tumor invasion and the presence of metastasis. Therefore, SRC, LYN and CKB expression or DNA methylation could be useful markers for predicting tumor progression and targeting in anti-cancer strategies.
Descrição: MELLO, Adriano Azevedo ; LEAL, Mariana Ferreira ; REY, Juan Antonio ; PINTO, Giovanny Rebouças ; LAMARÃO, Leticia Martins ; MONTENEGRO, Raquel Carvalho ; ALVES, Ana Paula Negreiros Nunes ; ASSUMPÇÃO, Paulo Pimentel ; BORGES, Barbara do Nascimento ; SMITH, Marília Cardoso ; BURBANO, Rommel Rodriguez. Deregulated expression of SRC, LYN and CKB kinases by DNA methylation and Its potential role in gastric cancer invasiveness and metastasis. Plos One, San Francisco, v. 10, p. 1-23, out. 2015.
URI: http://www.repositorio.ufc.br/handle/riufc/17731
ISSN: 1932-6203
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