Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/18153
Title in Portuguese: Rapid discrimination of klebsiella pneumoniae carbapenemase 2 – producing and non-producing klebsiella pneumoniae strains using near-infrared spectroscopy (NIRS) and multivariate analysis
Author: Marques, Aline S.
Moraes, Edgar P.
Ansaldi Júnior, Miguel Angel
Moura, Andrew D.
A. Neto, Valter F.
M. Neto, Renato
Lima, Kássio M. G.
Keywords: Klebsiella pneumoniae
Issue Date: Mar-2015
Publisher: Talanta
Citation: MARQUES, A. S. et al. Rapid discrimination of klebsiella pneumoniae carbapenemase 2 – producing and non-producing klebsiella pneumoniae strains using near-infrared spectroscopy (NIRS) and multivariate analysis. Talanta, London, v. 134, p. 126-131, mar. 2015.
Abstract: Klebsiella pneumoniae Carbapenemase (KPC–2)-producing and non-producing Klebsiella pneumoniae (KP) have rapidly disseminated worldwide, challenging the diagnostics of Gram-negative infections. We evaluate the potential of a novel non-destructive and rapid method based on Near-Infrared Spectroscopic (NIRS) and multivariate analysis for distinguishing KPC-2 – producing and non-producing KP. Thirty-nine NIRS spectra (24 KPC-2-producing KP, 15 KPC-2 non-producing KP) were acquired; different pre-processing methods such as baseline correction, derivative and Savitzky–Golay smoothing were performed. A spectral region fingerprint was achieved after using genetic algorithm–linear discriminant analysis (GA–LDA) and successive projection algorithm (SPA–LDA) algorithms for variable selection. The variables selected were then used for discriminating the microorganisms.Accuracy test results including sensitivity and specificity were determined. Sensitivity in KPC-2 producing and non-producing KP categories was 66.7% and 75%, respectively, using a SPA-LDA model with 66 wavenumbers. The resulting GA-LDA model successfully classified both microorganisms with respect to their “fingerprints” using only 39 wavelengths. Sensitivity in KPC-2 producing category was moderate(≈66.7%) using a GA-LDA model. However, sensitivity in KPC-2 non-producing category using GA-LDA accurately predicted the correct class (with 100% accuracy). As100% accuracy was achieved, this novel approach identifies potential biochemical markers that may have a relation with microbial functional roles and means of rapid identification of KPC-2 producing and non-producing KP strains.
URI: http://www.repositorio.ufc.br/handle/riufc/18153
metadata.dc.type: Artigo de Periódico
ISSN: 0039-9140 Print
1873-3573 On line
Appears in Collections:DCIR - Artigos publicados em revista científica

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