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Título: TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats
Autor(es): OLIVEIRA, Bruna Vasconcelos
SILVA, Paulo Goberlânio de Barros
NOJOSA, Jacqueline Santiago
BRIZENO, Luiz André Cavalcante
FERREIRA, Jamile Magalhães
SOUSA, Fabrício Bitú
MOTA, Mário Rogério Lima
ALVES, Ana Paula Negreiros Nunes
Palavras-chave: Diabetes Mellitus
Úlceras Orais
Data do documento: 2016
Editor: Journal of Applied Oral Science
Citação: OLIVEIRA, B. V. ; SILVA, P. G. B. ; NOJOSA, J. S. ; BRIZENO, L. A. C. ; FERREIRA, J. M. ; SOUSA, F. B. ; MOTA, M. R. L. ; ALVES, A. P. N. N. (2016)
Abstract: OBJECTIVES: Characterize the cell profile and immunostaining of proinflammatory markers in an experimental model of bisphosphonate-related osteonecrosis of the jaw (BRONJ). MATERIALS AND METHODS: Male Wistar rats (n = 6– 7) were treated chronically with saline solution or zoledronic acid (ZA) at 0.04, 0.20, and 1.00 mg kg 1 (1.4 3 10 7, 6.9 3 10 6, and 3.4 3 10 5 mol kg 1), and subsequently, the first left inferior molar was extracted. Were performed counting of viable and empty osteocyte lacunae, viable and apoptotic osteoclasts, polymorphonuclear neutrophil, mast cells (toluidine blue), and the positive presence cells for CD68, tumor necrosis factor-alpha (TNF-a), IL (interleukin)-1b, inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-kB) and IL-18 binding protein (IL-18 bp). RESULTS: BRONJ was showed in ZA treated with 0.20 and 1.00 mg kg 1. There is a dose dependent increase in percentage of empty osteocyte lacunae (P < 0.001) and apoptotic osteoclasts (P < 0.001), counting of total osteoclasts (P = 0.003), polymorphonuclear neutrophil cells (P = 0.009), cytoplasmic-positive cells of CD68 (P < 0.001), TNF-a (P = 0.001), IL-1b (P = 0.001), iNOS (P < 0.001), NF-kB (P = 0.006), and nuclear-positive cells of NF-kB (P = 0.011). Consequently, there is no difference in mast cells (P = 0.957), and IL-18 bp immunostaining decreases dose dependently (P = 0.005). CONCLUSIONS: BRONJ is characterized by increases in immunostaining for proinflammatory markers and NF-kB and inversely associated with cells exhibiting IL-18 bp.
Descrição: OLIVEIRA, B. V. et al. TNF-alpha expression, evaluation of collagen, and TUNEL of Matricaria recutita L. extract and triamcinolone on oral ulcer in diabetic rats. Journal of Applied Oral Science, Bauru, v. 24, n. 3, p. 278-290, 2016.
ISSN: 1678-7765 On line
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