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Title in Portuguese: The anti-inflammatory effects of N-methyl-(2S,4R)- trans -4-hydroxy- l -proline from Syderoxylon obtusifolium are related to its inhibition of TNF-alpha and inflammatory enzymes
Author: Aquino, Pedro Everson Alexandre de
Magalhães, Talita Rocha
Nicolau, Lucas Antônio Duarte
Leal, Luzia Kalyne A. Moreira
Aquino, Nayara Coriolano de
Santos, Sabrina Matias dos
Neves, Kelly Rose Tavares
Silveira, Edilberto Rocha
Viana, Glauce Socorro de Barros
Keywords: Estresse Oxidativo
Issue Date: Jan-2017
Publisher: Phytomedicine
Citation: AQUINO, P. E. A. de et al. The anti-inflammatory effects of N-methyl-(2S,4R)-trans-4-hydroxy-l-proline from Syderoxylon obtusifolium are related to its inhibition of TNF-alpha and inflammatory enzymes. Phytomedicine, Stuttgart, v. 24, p. 14-23, jan. 2017.
Abstract: Background Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn., Sapotaceae family, is a medicinal species native to the Brazilian Northeastern region. The plant is popularly used as an anti-inflammatory and hypoglycemic. Purpose To evaluate the anti-inflammatory properties of the N-methyl-(2S,4R)-trans-4-hydroxy-l-proline (NMP) from S. obtusifolium leaves in models of inflammation and to clarify its action mechanisms. Methods Male Swiss mice were distributed intocontrols and groups treated with NMP (25, 50 and 100 mg/kg, p.o.), indomethacin or morphine (reference drugs). The animals were subjected to the formalin, carrageenan-induced edema and peritonitis tests. Furthermore, peritoneal lavage and slices from edematous paws were used for histological and immunohistochemical (iNOS, TNF-alpha, COX-2 and NF-kB) assays. Results Decreases in licking time, in the 1st and mainly in the 2nd phases of the formalin test, were shown after NMP treatments. In addition, decreases (around 50%) in paw edema were noticed at the 3rd h. The HE staining of paw slices demonstrated a complete reversion of the increased PMN cell numberafter NMP treatment. Similarly, decreases higher than 70% were also demonstrated in PMN cells, in the peritoneal fluid. Furthermore, NMP significantly decreased iNOS, TNF-alpha, COX-2 and NF-kB immunoreactivities. Conclusions We showed that S. obtusifolium presents a potent anti-inflammatory activity, due to the presence of the N-methyl-(2S,4R)-trans-4-hydroxy-l-proline(NMP) in the plant extract. This action is related to the inhibition by NMP of TNF-alpha and inflammatory enzymes.
metadata.dc.type: Artigo de Periódico
ISSN: 0944-7113
1618-095X On line
Appears in Collections:DFAR - Artigos publicados em revistas científicas

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