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|Title in Portuguese:||Impact of testicular sperm extraction and testicular sperm aspiration on gonadal function in an experimental rat model|
|Author:||Barroso, Leocácio Venícius Sousa|
Cerqueira, João Batista Gadelha
Miranda, Eduardo P.
Alcantara, Rafael Jorge Alves de
Jamacaru, Francisco Vagnaldo F.
Moraes, Manoel Odorico de
Medeiros, Maria Angelina da Silva
Gonzaga-Silva, Lúcio Flávio
|Publisher:||International braz j urol|
|Citation:||BARROSO, Leocácio Venícius Sousa et al. Impact of testicular sperm extraction and testicular sperm aspiration on gonadal function in an experimental rat model. Int. braz j urol., Rio de Janeiro, v. 44, n.1, jan./feb. 2018.|
|Abstract:||Purpose: To assess the impact of sperm retrieval on the gonadal function of rats with impaired spermatogenesis by comparing testicular sperm extraction (TESE) to aspiration (TESA). The efficacy of these procedures to sperm obtainment was also compared. Materials and Methods: A pilot study showed impaired spermatogenesis, but normal testosterone (T) production after a bilateral orchidopexy applied to 26 rats, which were randomly assigned into four groups: TESE (n=7), TESA (n=7), SHAM (n=6) and Control (n=6). The T levels were measured through comparative analysis after the orchidopexy. Results: There was no statistical difference in the animal’s baseline T levels after orchidopexy in comparison to the controls: the TESE and TESA groups, 6.66±4.67ng/mL; the SHAM group (orchidopexy only), 4.99±1.96ng/mL; and the Control, 4.75±1.45ng/ mL, p=0.27. Accordingly, no difference was found in the postoperative T levels: TESE, 5.35±4.65ng/mL; TESA, 3.96±0.80ng/mL; SHAM, 3.70±1.27ng/mL; p=0.4. The number of sperm cells found through TESE (41.0±7.0) was significantly larger than that found through TESA (21.3±8.1, p=0.001). Moreover, higher tissue weight was found through TESE (0.09±0.02g versus 0.04±0.04g, p=0.04). Conclusions: The testicular sperm capture performed in rats through extraction or aspiration, after orchidopexy, did not significantly decrease the T levels. The amount of sperm found through testicular sperm extraction was higher than that through testicular sperm aspiration.|
|Appears in Collections:||DFIFA - Artigos publicados em revista científica|
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