Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/40333
Title in Portuguese: Antifungal activity of palmatine against strains of Candida spp. resistant to azoles in planktonic cells and biofilm
Author: Campos, Rosana de Sousa
Silva, Cecília Rocha da
Andrade Neto, João Batista de
Sampaio, Letícia Serpa
Nascimento, Francisca Bruna Stefany Aires do
Moraes, Manoel O. de
Cavalcanti, Bruno Coêlho
Magalhães, Hemerson Iury Ferreira
Gomes, Akenaton Onassis Cardoso Viana
Lobo, Marina Duarte Pinto
Nobre Júnior, Hélio Vitoriano
Keywords: Candida
Biofilmes
Biofilms
Citometria de Fluxo
Flow Cytometry
Ensaio Cometa
Comet Assay
Issue Date: 2018
Publisher: International Journal of Current Microbiology and Applied Sciences
Citation: CAMPOS, Rosana de Sousa et al. Antifungal activity of palmatine against strains of Candida spp. resistant to azoles in planktonic cells and biofilm. Int. J. Curr. Microbiol. App. Sci., v. 7, n. 2, p. 3657-3669, 2018.
Abstract: The incidence of fungal infections has increased and it is necessary to seek new antifungals that also act against resistant strains. Palmatine is a protoberberine alkaloid noteworthy for its various pharmacological properties, including antifungal properties. The objective of this study was to evaluate the activity of palmatine against Candida spp. strains resistant to azole in planktonic cells and biofilm, and to elucidate the possible mechanism of cell death. Palmatine showed an antifungal activity with MICs ranging from 32 to 128 μg/mL. In the formed biofilms, palmatine reduced by 50% cell viability at the concentration of 20x MIC (C. albicans) and 10x MIC (C. tropicalis and C. parapsilosis). In the formed biofilms, the same percentage of inhibition was achieved at a concentration of 1x MIC (C. albicans) and 2x MIC (C. tropicalis and C. parapsilosis). It was found that palmatine causes damage to DNA, promotes mitochondrial depolarization and increasing the externalization of phosphatidylserine, suggesting cell death by apoptosis. The antifungal action of palmatine in Candida spp. resistant to azoles in planktonic cells and biofilm formed and in formation, as well as the fact of not having presented cytotoxicity in mammalian cells, point to a promising new anti-candida molecule.
URI: http://www.repositorio.ufc.br/handle/riufc/40333
ISSN: Print 2319-7692
Online 2319-7706
Appears in Collections:DFIFA - Artigos publicados em revista científica

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