Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/40345
Title in Portuguese: Chemical composition and antifungal In vitro and in silico, antioxidant, and anticholinesterase activities of extracts and constituents of Ouratea fieldingiana (DC.) baill
Author: Nascimento, José Eranildo Teles do
Rodrigues, Ana LivyaMoreira
Lisboa, Daniele Silva de
Liberato, Hortência Ribeiro
Falcão, Maria José Cajazeiras
Silva, Cecília Rocha da
Nobre Júnior, Hélio Vitoriano
Braz Filho, Raimundo
Paula Junior, Valdir Ferreira de
Alves, Daniela Ribeiro
Morais, SeleneMaia de
Keywords: Ochnaceae
Candida
Flavonoides
Issue Date: Nov-2018
Publisher: Evidence-Based Complementary and Alternative Medicine
Citation: NASCIMENTO, José Eranildo Teles do et al. Chemical composition and antifungal In vitro and in silico, antioxidant, and anticholinesterase activities of extracts and constituents of Ouratea fieldingiana (DC.) baill. Evidence-Based Complementary and Alternative Medicine, v. 2018, p. 1-13, nov. 2018.
Abstract: Ouratea fieldingiana (Gardner) Engl is popularly used for wound healing. This study describes the main chemical compounds present in extracts of O. fieldingiana and evaluates their biological potential by investigating antifungal, antioxidant, and anticholinesterase activities.The actionmechanismofmain antifungal compound was investigated bymolecular docking using the enzyme sterol 14-𝛼 demethylase, CYP51, required for ergosterol biosynthesis. The seeds and leaves were extracted with ethanol in a Soxhlet apparatus and by maceration, respectively. Both extracts were subjected to silica gel column chromatography for isolation of main constituents, followed by purification in sephadex. The structures of compounds were established by 1H and 13C-NMR spectroscopy and identified by comparison with literature data as amentoflavone and kaempferol 3-O-rutinoside, respectively.The antioxidant activities of the extracts were determined by the DPPH and ABTS free radical inhibition methods. In general, the extracts with the highest antioxidant activity corresponded to those with higher content of phenolic compounds and flavonoids. Theethanol extracts and two isolated compounds presented relevant antifungal activity against several Candida strains.The in silico findings revealed that the compound amentoflavone coupled with the CYP450 protein due to the low energy stabilization (-9.39 kcal/mol), indicating a possible mechanism of action by inhibition of the ergosterol biosynthesis of Candida fungi.
URI: http://www.repositorio.ufc.br/handle/riufc/40345
ISSN: 1741-427X
1741-4288 (On line)
Appears in Collections:DFAR - Artigos publicados em revistas científicas

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