Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/48387
Title in Portuguese: Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes
Author: Farias, V. X.
Uchoa, P.N.
Aquino, C.P.
Britto, L.R.G.
Fonteles, M.C.
Leal-Cardoso, J.H.
Silva-Alves, K.S.
Havt, A.
Prata, M.M.G.
Heimark, D.B.
Nascimento, N.R.F.
Santos, C.F.
Keywords: Neuropatias Diabéticas
Diabetic Neuropathies
Inositol
Issue Date: Jun-2019
Publisher: Brazilian Journal of Medical and Biological Research
Citation: FARIAS, V. X. et al. Expression of myo-inositol cotransporters in the sciatic nerve and dorsal root ganglia in experimental diabetes. Braz J Med Biol Res, Ribeirão Preto, v. 52, n. 6, p. 1-10, jun. 2019.
Abstract: The transport of myo-inositol is the main mechanism for the maintenance of its high intracellular levels. We aimed to measure the mRNA and protein levels of myo-inositol cotransporters in the sciatic nerve (SN) and dorsal root ganglia (DRG) during experimental diabetes. Streptozotocin-induced (STZ; 4, 8, and 12 weeks; 65 mg/kg; ip) diabetic rats (DB) and age-matched euglycemic (E) rats were used for the analysis of mRNA and protein levels of sodium myo-inositol cotransporters 1, 2 (SMIT1, SMIT2) or H+/myo-inositol cotransporter (HMIT). There was a significant reduction in the mRNA levels for SMIT1 in the SN and DRG (by 36.9 and 31.0%) in the 4-week DB (DB4) group compared to the E group. SMIT2 was not expressed in SN. The mRNA level for SMIT2 was up-regulated only in the DRG in the DB4 group. On the other hand, the protein level of SMIT1 decreased by 42.5, 41.3, and 44.8% in the SN after 4, 8, and 12 weeks of diabetes, respectively. In addition, there was a decrease of 64.3 and 58.0% of HMIT in membrane and cytosolic fractions, respectively, in the SN of the DB4 group. In the DRG, there was an increase of 230 and 86.3% for SMIT1 and HMIT, respectively, in the DB12 group. The levels of the main inositol transporters, SMIT1 and HMIT, were greatly reduced in the SN but not in the DRG. SMIT-1 was selectively reduced in the sciatic nerve during experimental STZ-induced diabetes.
URI: http://www.repositorio.ufc.br/handle/riufc/48387
metadata.dc.type: Artigo de Periódico
ISSN: On-line 1414-431X
Appears in Collections:DFIFA - Artigos publicados em revista científica

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