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|Title in Portuguese:||Inhibition of metastatic potential of B16-F10 melanoma cell line in vivo and in vitro by bi fl orin|
|Author:||Carvalho, Adriana Andrade|
Costa, Patrícia Marçal da
Souza, Luciana Gregório da Silva
Lemos, Telma Leda G.
Alves, Ana Paula Negreiros Nunes
Pessoa, Cláudia Pessoa
Moraes, Manoel Odorico de
|Publisher:||Life sciences (Elmsford)|
|Citation:||CARVALHO, A. A. de et al. Inhibition of metastatic potential of B16-F10 melanoma cell line in vivo and in vitro by biflorin. Life Sciences, Elmsford, v. 93, n. 5-6, p. 201-207, ago. 2013.|
|Abstract:||Aim: The aim of this study was to determine the antimetastatic potential of bi fl orin using in vivo and in vitro approaches. Main methods: Bi fl orin was isolated from Capraria bi fl ora collected in Fortaleza, Ceará, Brazil. Adhesion, migra- tion and invasion assays were performed to avail of the antimetastatic potential of this quinone. Experimen- tal metastasis was performed to avail of the antimetastatic potential of bil fl orin using in vivo assay. Key fi ndings: Treatmentwithbi fl orin(25and50 mg/kg/day)wasshown tobeeffectiveinreducingB16-F10mel- anoma metastasis in C57BL/6 mice. The administration of bi fl orin at 25 mg/kg/day intraperitoneally inhibited the formationofmetastases byabout57%compared to untreatedcontrolanimals.When theanimals weretreat- edwith50 mg/kg/dayintraperitoneally,therewasa71%decreaseinthenumberoflungmetastases.Morpholog- ical assays showed the presence of hemosiderin and erythrocytes in the lung parenchyma, indicating the occurrence of hemorrhage, probably a side effect of bi fl orin. Bi fl orin at non-toxic concentrations (0.5, 1.0 and 1.5 g/mL) was tested directly on B16-F10 cells in vitro, and it inhibited cell adhesion to type I collagen and cell motility using the wound-healing assay. Signi fi cance: These data suggest that bi fl orin has a promising antimetastatic potential, as shown by its anti-adhesion, anti-migration and anti-invasion properties against a metastatic melanoma cell line. However, further studies are essential to elucidate its mechanism of action.|
1879-0631 On line
|Appears in Collections:||DFIFA - Artigos publicados em revista científica|
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