Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/59043
Title in Portuguese: Differential expression of cysteine peptidase genes in the inner integument and endosperm of developing seeds of Jatropha curcas L. (Euphorbiaceae)
Author: Rocha, Antônio J.
Soares, Emanoella L.
Costa, José H.
Costa, Washington L. G.
Soares, Arlete A.
Nogueira, Fábio C. S.
Domont, Gilberto B.
Campos, Francisco A. P.
Keywords: Programmed cell death
Protein processing
Oilseeds
Vacuolar processing enzyme
KDEL-tailed cysteine peptidase
Issue Date: 2013
Citation: ROCHA, Antônio J. et al. Differential expression of cysteine peptidase genes in the inner integument and endosperm of developing seeds of Jatropha curcas L. (Euphorbiaceae). Plant Science, [s.l.], v. 213, p. 30-37, 2013.
Abstract: In several plant tissues, programmed cell death (PCD) is mediated by the combined action of cysteine peptidases, namely KDEL-tailed cysteine peptidases (KDEL-CysEP) and vacuolar processing enzymes (VPE). Here, we performed a search of the draft genome of Jatropha curcas L. (Euphorbiaceae) and identified 2 genes for KDEL-CysEP (Jc-CysEP1 and Jc-CysEP2) and 3 genes for VPE (Jc-βVPE, Jc-γVPE and Jc-ıVPE) and determined the expression patterns of these genes by RT-qPCR in integument and cellular endosperm of seeds collected at seven different developmental stages. We were able to demonstrate that the expression of Jc-CysEP1, Jc-CysEP2, Jc-βVPE and Jc-γVPE proceeded rapidly from Stage IV, with Jc-CysEP2 displaying the highest relative expression; expression of Jc-ıVPE could not be detected in any of the tissues/developmental stages analyzed. Additionally, we showed that the expression pattern of these peptidases correlates with anatomical changes in integument and cellular endosperm, thus suggesting a role for both classes of peptidases in PCD and in protein processing, both of which occur simultaneously in each of these tissues.
URI: http://www.repositorio.ufc.br/handle/riufc/59043
metadata.dc.type: Artigo de Periódico
ISSN: 0168-9452
Appears in Collections:DBIO - Artigos publicados em revista científica

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