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Título: The hydrogen sulfide donor, Lawesson’s reagent, prevents alendronate-induced gastric damage in rats
Autor(es): Nicolau, L.A.D.
Silva, R.O.
Damasceno, S.R.B.
Carvalho, N.S.
Costa, N.R.D.
Aragão, K.S.
Barbosa, A.L.R.
Soares, P.M.G.
Souza, M.H.L.P.
Medeiros, J.V.R.
Palavras-chave: Alendronato
Sulfeto de Hidrogênio
Data do documento: Ago-2013
Editor: Brazilian Journal of Medical and Biological Research
Citação: NICOLAU, L.A.D. ; SILVA, R.O. ; DAMASCENO, S.R.B. ; CARVALHO, N.S. ; COSTA, N.R.D. ; ARAGÃO, K.S. ; BARBOSA, A.L.R. ; SOARES, P.M.G. ; SOUZA, M.H.L.P. ; MEDEIROS, J.V.R. (2013)
Abstract: Our objective was to investigate the protective effect of Lawesson’s reagent, an H2S donor, against alendronate (ALD)-induced gastric damage in rats. Rats were pretreated with saline or Lawesson’s reagent (3, 9, or 27 mmol/kg, po) once daily for 4 days. After 30 min, gastric damage was induced by ALD (30 mg/kg) administration by gavage. On the last day of treatment, the animals were killed 4 h after ALD administration. Gastric lesions were measured using a computer planimetry program, and gastric corpus pieces were assayed for malondialdehyde (MDA), glutathione (GSH), proinflammatory cytokines [tumor necrosis factor (TNF)-a and interleukin (IL)-1b], and myeloperoxidase (MPO). Other groups were pretreated with glibenclamide (5 mg/kg, ip) or with glibenclamide (5 mg/kg, ip)+diazoxide (3 mg/kg, ip). After 1 h, 27 mmol/kg Lawesson’s reagent was administered. After 30 min, 30 mg/kg ALD was administered. ALD caused gastric damage (63.35±9.8 mm2); increased levels of TNF-a, IL-1b, and MDA (2311±302.3 pg/mL, 901.9±106.2 pg/mL, 121.1±4.3 nmol/g, respectively); increased MPO activity (26.1±3.8 U/mg); and reduced GSH levels (180.3±21.9 mg/g). ALD also increased cystathionine-c-lyase immunoreactivity in the gastric mucosa. Pretreatment with Lawesson’s reagent (27 mmol/kg) attenuated ALD-mediated gastric damage (15.77±5.3 mm2); reduced TNFa, IL-1b, and MDA formation (1502±150.2 pg/mL, 632.3±43.4 pg/mL, 78.4±7.6 nmol/g, respectively); lowered MPO activity (11.7±2.8 U/mg); and increased the level of GSH in the gastric tissue (397.9±40.2 mg/g). Glibenclamide alone reversed the gastric protective effect of Lawesson’s reagent. However, glibenclamide plus diazoxide did not alter the effects of Lawesson’s reagent. Our results suggest that Lawesson’s reagent plays a protective role against ALD-induced gastric damage through mechanisms that depend at least in part on activation of ATP-sensitive potassium (KATP) channels.
Descrição: NICOLAU, L.A.D. ; SILVA, R.O. ; DAMASCENO, S.R.B. ; CARVALHO, N.S. ; COSTA, N.R.D. ; ARAGÃO, K.S. ; BARBOSA, A.L.R. ; SOARES, P.M.G. ; SOUZA, M.H.L.P. ; MEDEIROS, J.V.R. The hydrogen sulfide donor, Lawesson’s reagent, prevents alendronate-induced gastric damage in rats. Braz. J. Med. Biol. Res., Ribeirão Preto, v. 46, n.8, ago. 2013.
URI: http://www.repositorio.ufc.br/handle/riufc/7202
ISSN: 1414-431X Impresso
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