Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/7231
Title in Portuguese: (4-Methoxyphenyl)(3,4,5-trimethoxyphenyl)methanone inhibits tubulin polymerization, induces G2/M arrest, and triggers apoptosis in human leukemia HL-60 cells
Author: Magalhães, Hemerson I.F.
Wilke, Diego V.
Bezerra, Daniel P.
Cavalcanti, Bruno C.
Rotta, Rodrigo
Lima, Dênis P. de
Beatriz, Adilson
Moraes Filho, Manoel Odorico de
Diniz-Filho, Jairo
Pessoa, Claudia
Keywords: Apoptose
Tubulinos
Issue Date: Oct-2013
Publisher: Toxicology and Applied Pharmacology
Citation: MAGALHÃES, H. I. F. et al. (4-Methoxyphenyl)(3,4,5-trimethoxyphenyl) methanone inhibits tubulin polymerization, induces G2/M arrest, and triggers apoptosis in human leukemia HL-60 cells. Toxicology and Applied Pharmacology, San Diego, Calif., v. 272, n. 1, p. 117-126, out. 2013.
Abstract: (4-Methoxyphenyl)(3,4,5-trimethoxyphenyl)methanone (PHT) is a known cytotoxic compound belonging to the phenstatin family. However, the exact mechanism of action of PHT-induced cell death remains to be determined. The aim of this study was to investigate the mechanisms underlying PHT-induced cytotoxicity. We found that PHT displayed potent cytotoxicity in different tumor cell lines, showing IC50 values in the nanomolar range. Cell cycle arrest in G2/M phase along with the augmented metaphase cells was found. Cells treated with PHT also showed typical hallmarks of apoptosis such as cell shrinkage, chromatin condensation, phosphatidylserine exposure, increase of the caspase 3/7 and 8 activation, loss of mitochondrial membrane potential, and internucleosomal DNA fragmentation without affecting membrane integrity. Studies conducted with isolated tubulin and docking models confirmed that PHT binds to the colchicine site and interferes in the polymerization of microtubules. These results demonstrated that PHT inhibits tubulin polymerization, arrests cancer cells in G2/M phase of the cell cycle, and induces their apoptosis, exhibiting promising anticancer therapeutic potential.
URI: http://www.repositorio.ufc.br/handle/riufc/7231
metadata.dc.type: Artigo de Periódico
ISSN: 0041-008X Impresso
1096-0333 Online
Appears in Collections:DFIFA - Artigos publicados em revista científica

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