Use este identificador para citar ou linkar para este item: http://www.repositorio.ufc.br/handle/riufc/9472
Título: Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats
Autor(es): Guimarães Filho, Artur
Cunha, Rodrigo Maranguape Silva da
Vasconcelos, Paulo Roberto Leitão de
Guimarães, Sergio Botelho
Palavras-chave: Glutamina
Hepatectomia
Regeneração Hepática
Data do documento: Jun-2014
Editor: Acta Cirúrgica Brasileira
Citação: GUIMARÃES FILHO, A. ; CUNHA, R. M. S. ; VASCONCELOS, P. R. L. ; GUIMARÃES, S. B. (2014)
Abstract: PURPOSE: To evaluate the relative gene expression (RGE) of cytosolic (MDH1) and mitochondrial (MDH2) malate dehydrogenases enzymes in partially hepatectomized rats after glutamine (GLN) or ornithine alpha-ketoglutarate (OKG) suplementation. METHODS: One-hundred and eight male Wistar rats were randomly distributed into six groups (n=18): CCaL, GLNL and OKGL and fed calcium caseinate (CCa), GLN and OKG, 0.5g/Kg by gavage, 30 minutes before laparotomy. CCaH, GLNH and OKGH groups were likewise fed 30 minutes before 70% partial hepatectomy. Blood and liver samples were collected three, seven and 14 days after laparotomy/hepatectomy for quantification of MDH1/MDH2 enzymes using the real-time polymerase chain reaction (PCR) methodology. Relative enzymes expression was calculated by the 2-ΔΔCT method using the threshold cycle (CT) value for normalization.RESULTS: MDH1/MDH2 RGE was not different in hepatectomized rats treated with OKG compared to rats treated with CCa. However, MDH1/MDH2 RGE was greater on days 3 (321:1/26.48:1) and 7 (2.12:1/2.48:1) while MDH2 RGE was greater on day 14 (7.79:1) in hepatectomized rats treated with GLN compared to control animals. CONCLUSION: Glutamine has beneficial effects in liver regeneration in rats by promoting an up-regulation of the MDH1 and MDH2 relative gene expression.
Descrição: GUIMARÃES FILHO, Artur ; CUNHA, Rodrigo Maranguape Silva da ; VASCONCELOS, Paulo Roberto Leitão de ; GUIMARÃES, Sergio Botelho. Glutamine and ornithine alpha-ketoglutarate supplementation on malate dehydrogenases expression in hepatectomized rats. Acta Cirúrgica Brasileira, São Paulo, v. 29, n. 6, jun., 2014.
URI: http://www.repositorio.ufc.br/handle/riufc/9472
ISSN: 1678-2674 Online
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