Please use this identifier to cite or link to this item: http://www.repositorio.ufc.br/handle/riufc/8541
Title in Portuguese: Synergistic effect of the flavonoid catechin, quercetin, or epigallocatechin gallate with fluconazole induces apoptosis in Candida tropicalis resistant to fluconazole
Author: Silva, Cecília Rocha da
Andrade Neto, João Batista de
Campos, Rosana de Sousa
Figueiredo, Narjara Silvestre
Sampaio, Letícia Serpa
Magalhães, Hemerson Iury Ferreira
Cavalcanti, Bruno Coêlho
Gaspar, Danielle Macêdo
Andrade, Geanne Matos de
Lima, Iri Sandro Pampolha
Viana, Glauce Socorro de de Barros
Moraes, Manoel Odorico de
Lobo, Marina Duarte Pinto
Grangeiro, Thalles Barbosa
Nobre Júnior, Hélio Vitoriano
Keywords: Flavonóides
Catequina
Quercetina
Issue Date: Mar-2014
Citation: SILVA, C. R. da et al. Synergistic Effect of the flavonoid catechin, quercetin, or epigallocatechin gallate with fluconazole induces apoptosis in Candida tropicalis resistant to fluconazole. Antimicrobial Agents and Chemotherapy, v. 58, n. 3, p. 1468-1478, mar. 2014.
Abstract: Flavonoids are a class of phenolic compounds commonly found in fruits, vegetables, grains, flowers, tea, and wine. They differ in their chemical structures and characteristics. Such compounds show various biological functions and have antioxidant, antimicrobial, anti-inflammatory, and antiapoptotic properties. The aim of this study was to evaluate the in vitro interactions of flavonoids with fluconazole against Candida tropicalis strains resistant to fluconazole, investigating the mechanism of synergism. Three combinations formed by the flavonoids ( )-catechin hydrated, hydrated quercetin, and ( )-epigallocatechin gallate at a fixed concentration with fluconazole were tested. Flavonoids alone had no antifungal activity within the concentration range tested, but when they were used as a cotreatment with fluconazole, there was significant synergistic activity. From this result, we set out to evaluate the possible mechanisms of cell death involved in this synergism. Isolated flavonoids did not induce morphological changes or changes in membrane integrity in the strains tested, but when they were used as a cotreatment with fluconazole, these changes were quite significant. When evaluating mitochondrial damage and the production of reactive oxygen species (ROS) only in the cotreatment, changes were observed. Flavonoids combined with fluconazole were shown to cause a significant increase in the rate of damage and the frequency of DNA damage in the tested strains. The cotreatment also induced an increase in the externalization of phosphatidylserine, an important marker of early apoptosis. It is concluded that flavonoids, when combined with fluconazole, show activity against strains of C. tropicalis resistant to fluconazole, promoting apoptosis by exposure of phosphatidylserine in the plasma membrane and morphological changes, mitochondrial depolarization, intracellular accumulation of ROS, condensation, and DNA fragmentation.
URI: http://www.repositorio.ufc.br/handle/riufc/8541
ISSN: 0066-4804 Print
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